Engineering Questions with Answers - Multiple Choice Questions
Separation Processes MCQ ‘s – Introduction to Bioseparations
1 - Question
Which of the following is not a classification of biological species?
a) Bio-molecules
b) Small molecules
c) Bio-Polymers
d) Cellular particulates
View Answer
Explanation: Bio-molecules are fats, carbohydrates, proteins etc. They are not species.
2 - Question
What are primary metabolites?
a) Synthesized during primary phase of cell growth
b) Synthesized during secondary phase of cell growth
c) Synthesized during death phase of cell growth
d) Synthesized during growth phase of cell
View Answer
Explanation: Primary metabolites include the amino acids and the disaccharides and vitamins that are synthesized during primary phase of cell growth.
3 - Question
What are secondary metabolites?
a) Synthesized during primary phase of cell growth
b) Synthesized during secondary phase of cell growth
c) Synthesized during the stationary phase
d) Synthesized during growth phase of cell
View Answer
Explanation: Secondary metabolites include steroids, cytoxins etc. which are synthesized during stationary phase of a cell.
4 - Question
What is the molecular weight range of proteins?
a) 1000 DA- 10000 DA
b) 10000 Da- 100000 Da
c) 100000 Da-1000000 Da
d) 100000Da – 10000000 Da
View Answer
Explanation: The size of a protein molecule is 10000 Da to 100000 Da.
5 - Question
What are nucleotides?
a) Nitrogenous bases covalently bonded to triose sugar
b) Nitrogenous bases covalently bonded to quadrose sugar
c) Nitrogenous bases covalently bonded to pentose sugar
d) Nitrogenous bases covalently bonded to hexose sugar
View Answer
Explanation: Nucleotides are nitrogenous bases covalently bonded topentose sugar. Example: in case of DNA, the sugar is ribose.
6 - Question
Which of the following is an incorrect difference between prokaryotic and eukaryotic cell?
a) Eukaryotic cell has a cell wall while prokaryotic does not
b) Eukaryotic cell has less developed nucleus than prokaryotic cells
c) Prokaryotic cell can be aerobic while eukaryotic cell is mostly anaerobic
d) Prokaryotic cell does not have membrane bound organelles while eukaryotic cell has membrane bound organelles
View Answer
Explanation: Prokaryotic cells can be anaerobic as well. Eukaryotes are mostly aerobic.
7 - Question
If the bioproduct mass is 113Kg and the impurities are 500g, what is the purity of the bioproduct?
a) 99.12%
b) 99.55%
c) 0.004%
d) 98.68%
View Answer
Explanation: Bioproduct purity = Bioproduct mass/ (bioproduct mass + purity).
8 - Question
If the mass fed to the separation process was 460Kg and the mass recovered was 422Kg, what is the % yield?
a) 93.77%
b) 8.2%
c) 91,73 %
d) 9%
View Answer
Explanation: Yield = Mass of bioproduct fed/ mass of bioproduct recovered.
9 - Question
What is the purpose of solute- solute separations and what are the species removed?
a) Purpose: clarify target species; Removes: culture media, fermentation broth
b) Purpose: extract target from cells; removes: small molecules, proteins
c) Purpose: Preserve target species ; Removes: Buffer solutions
d) Purpose: Fractionate target species; Removes: unrelated solutes ,small molecules.
View Answer
Explanation: The solute -solute separations are used to fractionate target species and remove small molecules like lipids, proteins etc
10 - Question
What is the purpose of fluid-solid separations and what are the species removed?
a) Purpose: clarify target species; Removes: culture media, fermentation broth
b) Purpose: extract target from cells; removes: small molecules, proteins
c) Purpose: Preserve target species ; Removes: Buffer solutions
d) Purpose: Fractionate target species; Removes: unrelated solutes ,small molecules.
View Answer
Explanation: The purpose of the fluid solid separations is to remove culture media and clarify target species.
11 - Question
Which of the following process is not employed to distinguish between contaminants and the product based on physiochemical features?
a) Filtration
b) Batch adsorption
c) Isoatachoporesis
d) Crystallization
View Answer
Explanation: Crystallization is not based on physiochemical features.
12 - Question
What is the overall percentage yield for penicillin manufacturing?
a) 20%
b) 30%
c) 40%
d) 50%
View Answer
Explanation: The yield is only 20% for starting materials.
13 - Question
Which of the following is also a process for penicillin recovery?
a) Adsorption on activated carbon
b) Direct crystallization
c) Degumming
d) Distillation
View Answer
Explanation: Penicillin can be recovered alternatively by adsorption on activated carbon.
14 - Question
What is the use of batch electrophoresis in procedure of purification of bioproduct?
a) It gives high resolution carbohydrates
b) It purifies completely
c) It gives high resolution protein content
d) The targeted product is concentrated by batch electrophoresis
View Answer
Explanation: Batch electrophoresis gives a high resolution of protein ingredients on a laboratory scale.