Engineering Questions with Answers - Multiple Choice Questions

MCQs on Transgenic Plants

1 - Question

. The process of expression of foreign genes in a plant is called___________
a) Gene expression
b) Transgenesis
c) Genetic transformation
d) Cell hybridization

View Answer

Answer: c
Explanation: Genetic transformation is the process of introducing, stably integration and expression of foreign genes in the plant. This whole process produces transgenic plants.

2 - Question

. In transgenesis, only cloned genes are introduced into the donor.
a) True
b) False

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Answer: a
Explanation: Unlike conventional breeding, only those genes are introduced in the plant which has agronomic importance, i.e., cloned genes without the cotransfer of the other undesirable gene.

3 - Question

. Name the strategy where two-plasmid system is used for the introduction of the gene?
a) Binary vector system
b) Co-integration vector strategy
c) Agrobacterium
d) Selectable marker strategy

View Answer

Answer: a
Explanation: In Binary vector system, two plasmids are used to introduce t-DNA into the plants. Both plasmids complement each other when present together in a system.

4 - Question

Which of the following is considered as a visual marker?
a) Antibiotic marker
b) Resistance marker
c) Selectable marker
d) Screenable marker

View Answer

Answer: d
Explanation: Screenable marker gene is used for determining transformation efficiency, confirming transformation, and monitoring gene or protein activity.

5 - Question

Name the marker gene of streptomycin?
a) HPT
b) SPT
c) PAT
d) BXN

View Answer

Answer: b
Explanation: Selectable marker gene is used to confer resistance to antibiotics i.e., SPT is used for streptomycin, HPT is for hygromycin, and BXN is for bromoxynil.

6 - Question

Name the first transgenic virus resistant plant?
a) Rice
b) Cotton
c) Tobacco
d) Tomato

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Answer: c
Explanation: In 1986, tobacco was produced using virus resistance gene, i.e., tobacco mosaic virus (TMV) coat protein, which generates resistance against virus attack.

7 - Question

Which of the following is supplemented with vitamin A in order to improve its nutritional quality?
a) Cotton
b) Potato
c) Tomato
d) Rice

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Answer: d
Explanation: The nutritional quality of rice has been improved by the addition of vitamin A (beta-carotene). This genetically engineered rice is called golden rice.

8 - Question

Name the enzyme which has silenced to delay the ripening process?
a) Polygalacturonase
b) Glyphosate
c) ADA
d) P53

View Answer

Answer: a
Explanation: Polygalacturonase is an enzyme which is responsible for ripening. By antisense technology, the gene which encodes for this enzyme has been silenced.

9 - Question

. Name the technique which is used to enhance the life of a tomato.
a) Antisense technology
b) In vitro gene transfer
c) Ex vivo gene transfer
d) Molecular farming

View Answer

Answer: a
Explanation: In antisense technology, an antisense copy of the gene is expressed in the tomato which silenced polygalacturonase enzyme.

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