Engineering Questions with Answers - Multiple Choice Questions
MCQs on DNA Replication
. Double-helix structure of DNA is discovered by___________
a) Gobind Khurana
c) Watson and Crick
Explanation: In 1953, Watson and Crick worked out on the double – helix structure of DNA and find out the complementary nature of two strands.
Base sequence of each parental strand considered to synthesis new complementary strand.
Explanation: The two strands of DNA are separated without breakage of a covalent bond and its base sequence act as the template strand to synthesize a new complementary strand.
What is a mode of replication in E.coli?
Explanation: Meselson and Stahl in 1958 conducted an experiment and demonstrated the semiconservative replication of DNA in E.coli.
What is the origin of replication?
a) Particular site at which DNA replication starts
b) Site which prevents initiation
c) Random location on the DNA
d) Site at which replication terminated
Explanation: Origin of replication is particular sites on DNA as replication does not start at random sites. Replication starts from a particular site and proceeds bidirectionally or unidirectionally till the terminus site.
How many numbers of replicon is found in E.coli?
a) Five replicon
b) Two replicon
c) Single replicon
d) Multiple replicon
Explanation: E.coli is monorepliconic and have single replicon while eukaryotic cells contain many replication origins on a single chromosome and called multirepliconic.
Which of the following protein does not involve in the initiation of replication?
b) SSB (Single strand binding protein)
Explanation: In eukaryotic replication, more than 20 proteins take part out of that the initiation of replication takes place in the presence of helicase, primase, SSB, DnaC, DnaA.
Which of the following protein is required for connecting Okazaki fragments?
a) Scaffold protein
d) DNA gyrase
Explanation: After initiation, the chain elongation and joining of Okazaki fragments take place by DNA gyrase, DNA ligase, DNA polymerase.
Name the protein, which is used for termination of replication?
c) Tus protein
d) DNA polymerase
Explanation: Tus protein is terminus binding protein which is used for termination of replication.
Which of the following would relax negatively supercoiled DNA?
a) DNA gyrase
b) Type I topoisomerase
Explanation: Negatively supercoiled DNA can be relaxed by topoisomerase I. It creates the nick in one strand of DNA and rotates freely to resolve supercoiling.
Name the protein, which is responsible for the formation of RNA primer?
Explanation: RNA primers are generated by using protein, primase which is also known as RNA polymerase. Primers are short RNA sequences.