Engineering Questions with Answers - Multiple Choice Questions
MCQs on Animal Cell Culture
1 - Question
The growth of animal cells in vitro in a suitable culture medium is called___________
a) Gene expression
b) Transgenesis
c) Plant tissue culture
d) Animal cell culture
View Answer
Answer: d
Explanation: Animal cell culture is the process of growing cell in vitro in a suitable culture medium which contains a mixture of nutrients and growth factors.
2 - Question
Cells from kidney tissues cannot survive independently, it requires other surface for attachment and survival.
a) True
b) False
View Answer
Answer: a
Explanation: Cells derived from kidney tissues are anchorage dependent primary cell culture which requires another surface for attachment and survival.
3 - Question
Name the type of culture which is prepared by inoculating directly from the tissue of an organism to culture media?
a) Primary cell culture
b) Secondary cell culture
c) Cell lines
d) Transformed cell culture
View Answer
Answer: a
Explanation: Primary cell culture is the direct inoculation of the tissues of an organism into a culture medium without going prior to cell proliferation.
4 - Question
. What is a cell line?
a) Multilayer culture
b) Transformed cells
c) Multiple growth of cells
d) Sub culturing of primary culture
View Answer
Answer: d
Explanation: Cell line is defined as the sub culturing of primary cell culture. It can be a finite cell line or a infinite cell line
5 - Question
Name the organism on which first cell line was observed?
a) E.coli
b) Sheep
c) Mouse
d) Drosophila
View Answer
Answer: c
Explanation: First cell line observed was mouse fibroblast L cell which was derived from connective tissues of mouse by exposing it to a chemical carcinogen.
6 - Question
Which of the following is the characteristics of a normal cell?
a) Anchorage independent
b) Continuous cell lines
c) Dependent on external growth factor
d) No contact inhibition
View Answer
Answer: c
Explanation: A normal cell needs external factors for its survival. It is anchorage dependent and have a finite number of cell lines.
7 - Question
Name the cell line of the human embryonic lung?
a) HeLa
b) WISH
c) L
d) MRC-5
View Answer
Answer: d
Explanation: MRC-5 is the cell line of the human embryonic lung while HeLa is the cell line of human cervical carcinoma. L represents human connective tissue and WISH is the cell line of human amnion.
8 - Question
Which of the following is NOT the part of growth medium for animal culture?
a) Starch
b) Serum
c) Carbon source
d) Inorganic salts
View Answer
Answer: a
Explanation: Growth medium of an animal cell contains serum (5-20%), nitrogen sources, inorganic salts, carbon sources, growth factors, buffer in water and vitamins.
9 - Question
Which of the following is NOT the major function of the serum?
a) Promotion of tuber and bulb formation
b) Stimulate cell growth
c) Enhance cell attachment
d) Provide transport proteins
View Answer
Answer: a
Explanation: Serum is the primary component of growth medium which helps in cell attachment and growth. It also provides transport protein for carrying hormone, lipids, and minerals.